For the first time in two years, the interim meeting went presential,
more precisely hybrid. The M30 meeting was held past 8th & 9th
November at the INL in Braga.
A total of 34 participants attended the meeting, 18 of them online.
• 8th November: Coordination
and Communication issues were addressed by Eva Balsa-Canto (IIM-CSIC) and Uxía
Vázquez (INXENIA), followed by Elena Couñago (CETMAR) who presented the first
results of the tasting trial developed with the collaboration of Fundación
Dorzán. Afterwards, short presentations were made on behalf of BENBOA, LANC,
IPMA, IIM-CSIC and IRMRS to give an update of the last developments made
regarding the obtention of ingredients and the production of the RTE product.
Paco Teira (BENBOA) offered a sample of the fillet with sauces to the partners
and Manuel López (NOTPLA) showed the sachets that will be included in the final
product. Finally, Uxía Vázquez (INXENIA) presented preliminary results on the
carbon footprint of the SEAFOOD-AGE product and the progress made regarding
packaging was presented by Maria de Sousa (UCC), Marta Lopez (IIM-CSIC) and Manuel
• 9th November: During the morning the 2nd training session addressed to the partners took place. This session, organised by Rozenn Le Vaillant (TQC) counted on the participation of Harri Määttä (OUAS), Ina Bremenkamp (UCC), Manuel Lopez (NOTPLA) and Hayley Alter (LANC), who gave didactic and demonstrative speeches on aspects related to packaging and smart predictive labels. The meeting finished with the participation of Carmen G. Sotelo, who talked about labelling and traceability. To wrap up, the project coordinator made a review of the project progress and invited to debate on the call for extension.
A new article related to the detection of E. coli O157 has been
accepted. On this occasion, the INL researchers have developed an isothermal
technique (RPA) combined with a sample treatment that allows the detection of
the bacteria on the same day the analysis is started, being able to see the
result with the naked eye by color change, or with the help of a UV lamp.
This new article is added to others that INL has been publishing throughout SEAFOOD-AGE project with the support of FEDER funds through the INTERREG Atlantic Area Programme:
During the 1st training session organised by TQC on 4th November, the Portuguese Institute of Sea and Atmosphere (IPMA) showed the different methods to assess the bioactivity of ingredients. This action was intended to familiarize the partners with techniques and processes that can be carried out at lab. IPMA’s presentation was supported by a series of video tutorials developed by them in the framework of SEAFOOD-AGE project.
Now, these videos are available through SEAFOOD-AGE
Youtube channel for any researcher or technician interested in these techniques:
Our partners, the Portuguese Institute for the Sea and Atmosphere (IPMA) together with the Marine Research Institute (IIM-CSIC), have just published their first Open access paper in the framework of the project.
Abstract: Fish discards and by-products can be
transformed into high value-added products such as fish protein hydrolysates
(FPH) containing bioactive peptides. Protein hydrolysates were prepared from
different parts (whole fish, skin and head) of several discarded species of the
North-West Spain fishing fleet using Alcalase. All hydrolysates had moisture
and ash contents lower than 10% and 15%, respectively. The fat content of FPH
varied between 1.5% and 9.4% and had high protein content (69.8–76.6%). The
amino acids profiles of FPH are quite similar and the most abundant amino acids
were glutamic and aspartic acids. All FPH exhibited antioxidant activity and
those obtained from Atlantic horse mackerel heads presented the highest
2,2-diphenyl-1- icrylhydrazyl (DPPH) radical scavenging activity, reducing
power and Cu2+ chelating activity. On the other hand, hydrolysates from gurnard
heads showed the highest ABTS radical scavenging activity and Fe2+ chelating
activity. In what concerns the _-amylase inhibitory activity, the IC50 values
recorded for FPH ranged between 5.70 and 84.37 mg/mL for blue whiting heads and
whole Atlantic horse mackerel, respectively. _-Glucosidase inhibitory activity
of FPH was relatively low but all FPH had high Angiotensin Converting Enzyme
(ACE) inhibitory activity. Considering the biological activities, these FPH are
potential natural additives for functional foods or nutraceuticals.
partner, the International Iberian Nanotechnology Laboratory, is working to
improve food safety. They have just published a novel multiplex real-time RPA
method for the rapid detection of Listeria monocytogenes:
development of novel methods for the rapid detection of Listeria monocytogenes
is of high interest due to due to particular concerns about this its ubiquity,
resistance to sanitation processes and high mortality rates resulting from
evaluation of a novel multiplex real-time Recombinase Polymerase Amplification
(RPA) method including an internal amplification control is reported in the
paper released in volume 92 of Food Microbiology. The method performance was
compared to that of the European reference method (ISO 11290-1) for the
detection of the species in samples from 40 commercial dairy products. A limit
of detection below 10 cfu/25 g or mL sample was achieved, and values higher
than 90% were obtained for relative sensitivity, specificity, accuracy,
positive and negative predictive values and the index (kappa) of concordance.
Analysis was achieved within one working day, compared to the six days required
using the ISO method. Moreover, slight modification of the ISO 11290-1 method
to include secondary enrichment in half Fraser broth resulted in the
confirmation of all positive samples.
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